Development of an indirect ELISA-NcSRS2 for detection of Neospora caninum antibodies in cattle
نویسندگان
چکیده
منابع مشابه
Comparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep Comparação entre ELISA baseado no antígeno recombinante NcSRS2 e RIFI para detecção de anticorpos de Neospora caninum em ovinos
Neospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were subjected to IFAT and rNcSRS2 based-...
متن کاملdevelopment and standardization of dot - elisa for detection of neospora caninum antibodies in cattle and comparison with standard indirect elisa and direct agglutination test (dat).
background: neospora caninum is a protozoan parasite from phylum apicomplexa and an important agent causing abortion in cattle which produce notable economic loss all around the world. methods: dot-elisa was set up performing checker board procedure and then 178 sera of cattle examined with commercial indirect elisa and direct agglutination test (dat) were also evaluated by dot-elisa afterwards...
متن کاملDevelopment and Standardization of Dot – ELISA for Detection of Neospora caninum Antibodies in Cattle and Comparison with Standard Indirect ELISA and Direct Agglutination Test (DAT)
BACKGROUND Neospora caninum is a protozoan parasite from phylum apicomplexa and an important agent causing abortion in cattle which produce notable economic loss all around the world. METHODS Dot-Elisa was set up performing checker board procedure and then 178 sera of cattle examined with commercial indirect ELISA and direct agglutination test (DAT) were also evaluated by dot-ELISA afterwards...
متن کاملDetection of anti-Neospora caninum antibodies in Iranian native cattle
Neospora caninum is an Apicomplexan parasite which may cause abortion in cattle. This study investigated occurrences of antibodies against N. caninum in Iranian native cattle. From September 2010 to September 2011, blood samples (n=768) of native cows were collected randomly from different rural regions of Hamedan (n=400) and Kurdistan provinces (n=368) located to the western part of Iran. All ...
متن کاملDevelopment of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle
In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (D...
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ژورنال
عنوان ژورنال: Veterinary Parasitology
سال: 2011
ISSN: 0304-4017
DOI: 10.1016/j.vetpar.2010.11.026